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คณะเกษตรศาสตร์ มหาวิทยาลัยเชียงใหม่

 

  ISSN: 0857-0841 (Print)
  ISSN: 2630-029X (Online)
 


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"Article Journal of Agriculture"
Volume 35 Issue 1 January 2019 , ISSN 0857-0841
Title. : Detection of Viruses in Melon and Cantaloupe Planted in Chiang Mai, Chiang Rai and Lamphun Provinces
Author : Salit Supakitthanakorn, and On-Uma Ruangwong
Page : 75-85
index word : ไวรัส (Virus), เมลอน (melon), แคนตาลูป (cantaloupe), ELISA , กล้องจุลทรรศน์อิเล็กตรอนแบบส่องผ่าน (transmission electron microscope)
Abstract:
Collection of melon and cantaloupe leaf samples showing virus disease symptoms were carried out in the production areas both greenhouse and open field in Chiang Mai, Chiang Rai and Lamphun provinces of northern Thailand. Enzyme-linked immunosorbent assay (ELISA) with monoclonal and polyclonal antibodies were used for virus detection. The results showed that after virus detection from total 163 leaves samples, the highest disease incidence was 68.87% caused by Melon yellow spot virus (MYSV) followed by the genus Geminivirus (4.9%), Papaya ringspot virus (PRSV) (2.4%) and Zucchini yellow mosaic virus (ZYMV) (2.4%). While Begomovirus and Watermelon silver mottle virus (WSMoV) were minimally detected with 1.8% and 1.2%, respectively. MYSV co-infected with Geminivirus were the highest incidence of mixed infections and followed by Begomovirus co-infected with Geminivirus. Triple virus infection (PRSV, WSMoV and ZYMV) was detected from cantaloupe leaf. Virus particles were observed by using transmission electron microscope (TEM), the result showed that the average size of enveloped nearly-spherical particles of MYSV with 80-150 nm diameter, flexuous particle of PRSV with 650-850 nm long and twinned-icosahedral particles of Geminivirus with 25 x 35 nm diameter. The results indicated that MYSV was the most threatening virus for melon and cantaloupe production in northern Thailand under both greenhouse and field conditions.
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